产品名称

Nitroreductase from Escherichia coli

一般描述

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

含 量

≥90% (SDS-PAGE), recombinant, expressed in E. coli

分子量

monomer mol wt 24000

MDL   number

MFCD03457727

适用范围

生物试剂

l  理化信息

外 观

淡黄色到黄色的粉末

l  生物学信息

生物活性/药理作用

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

应 用

Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphylococcus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment.It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.

样品准备说明

Produced using animal component-free materials.

有效单位定义

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37°C.

l  存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov