产品名称

Ascorbate Oxidase from Cucurbita sp.

一般描述

Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid.

别 称

L-Ascorbate:oxygen oxidoreductase,  Ascor

含 量

1,000-3,000 units/mg protein

CAS  NO.

9029-44-1

MDL  number

MFCD00130560

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

应 用

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study  the heat stress response of Arabidopsis .

样品准备说明

The enzyme is reconstituted in 4 mM sodium phosphate buffer, pH 5.6, with 0.05% (w/v) bovine serum albumin for the Sigma  enzymatic activity assay.

有效单位定义

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

l  存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov