产品名称

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

一般描述

D-lactic dehydrogenase catalyzes the conversion of D-lactate into D-pyruvate while reducing NAD₊ to NADH and H₊.

别 称

(R)-3-Hydroxybutanoate:NAD₊ oxidoreductase, 3-HBDH

含 量

≥200 units/mg protein

CAS  NO.

9028-38-0

MDL  number

MFCD00130633

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate  to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle  (Krebs cycle).

应 用

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H.,  and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd  edition, 4, 1836 (1974).

有效单位定义

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

l  包装与存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov