生物活性/药理作用 | Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm.Catalyzes the oxidization of primary alcohols to aldehydes. Molecular Weight: ~600 kDa Optimum pH: 8.5 pH Range: 5.5-8.5 Km values with the following substrates: Allyl alcohol 33.3 mM Benzyl alcohol 26.6 mM Ethanol 16.2 mM Formaldehyde 2.6 mM Methanol 2.15 mM N-Butanol 166 mM n-Propanol 66 mM Cofactor: FAD Inhibitors: 1,4-butynediol (irreversible),
propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H2O2, hydroxylamine, KBr,
KCN, methanol (substrate inhibitor), NaN3, PCMB, propynal, urea,
4-chloromercuribenzoic acid |
应 用 | Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes.It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. |
