产品名称

Alcohol Oxidase from Candida boidinii

别 称

Alcohol:oxygen oxidoreductase

含 量

5-15 units/mg protein

CAS  NO.

9073-63-6

MDL  number

MFCD00130452

适用范围

生物试剂

l  理化信息

外 观

黄色至橙色冻干粉末

溶解性(25℃)

100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)

物理形态

Contains potassium phosphate buffer salts, DTE, and stabilizer

l  生物学信息

生物活性/药理作用

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but  is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is  6.5-8.5, with the optimum pH being 7.5.

应 用

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder.

样品准备说明

Alcohol oxidase is soluble (1 mg protein/ml) in cold 100 mM potassium phosphate, pH 7.5, at 25°C. Solutions should be prepared just before use.

有效单位定义

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

l  包装与存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov