产品名称

Choline Oxidase from Arthrobacter globiformis

别 称

Choline: oxygen 1-oxidoreductase

含 量

8-20 units/mg solid

CAS  NO.

9028-67-5

MDL  number

MFCD00130787

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.

应 用

Choline oxidase from Sigma has been used in  the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.

样品准备说明

Solutions of choline oxidase may be prepared in 10 mM Trizma^®-HCl, pH 8.0, with 2.0 mM EDTA and 134 mM KCl.9 Choline oxidase solutions may also be prepared in 200mM Trizma^® -HCl, pH 8.0.6 Another publication cites preparation of 2 mg/mL stock solutions of choline oxidase in carbonate buffer, pH 9.0.

有效单位定义

One unit will form 1 μmole of H₂O₂ with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H₂O₂ are produced for every μmole of choline.

l  存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov