产品名称

Xanthine Oxidase (XOD) from cow milk

一般描述

Xanthine:oxygen oxidoreductase

含 量

~1 units/mg protein (At 25 °C with xanthine as the substrate.)

杂 质

<0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the  substrate) Note: Chromatographically  purified.

最佳PH

8.5-9.0

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8.

l  生物学信息

生物活性/药理作用

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

应 用

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

样品准备说明

Activator: O²

Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

有效单位定义

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E^293nm = 12.2 mmol _-1 x L x cm_-1)  from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

l  包装与存储

存储温度

-20℃

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov