产品名称

Cholesterol Oxidase microbial

别 称

Cholesterol: oxygen oxidoreductase

含 量

≥10 units/mg protein recombinant

CAS  NO.

9028-76-6

分子量

mol wt 55 kDa

MDL  number

MFCD00130783

适用范围

生物试剂

l  理化信息

外 观

冻干粉

溶解性(25°C)

50 mM potassium phosphate buffer, pH 7.0: soluble

l  生物学信息

生物活性/药理作用

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H²O². Optimum pH of the enzyme is 7.0. Hg₂₊, Ag₊, ionic detergents inhibit the enzyme activity.

应 用

Cholesterol oxidase is used to determine serum cholesterol.The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

样品准备说明

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

有效单位定义

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase ed system.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov