产品名称

Cholesterol Oxidase from Streptomyces sp.

别 称

Cholesterol: oxygen oxidoreductase

含 量

≥20 units/mg protein

CAS  NO.

9028-76-6

分子量

mol wt ~34 kDa

MDL  number

MFCD00130783

适用范围

生物试剂

l  理化信息

外 观

冻干粉

溶解性(25°C)

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

物理形态

lyophilized powder containing bovine serum albumin and sugars as stabilizers

l  生物学信息

生物活性/药理作用

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H²O².

应 用

Cholesterol oxidase from Streptomyces has been used in a study to assess the relationship between the micellar structure of model bile and the activity of esterase. Cholesterol oxidase from Streptomyces has also been used in a study to investigate the effects of sphingomyelin degradation on cell cholesterol oxidizability and steady-state distribution between the cell surface and the cell interior.Cholesterol oxidase is used to determine serum cholesterol.The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

样品准备说明

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use

有效单位定义

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov