产品名称

Cholesterol Oxidase from microorganisms

别 称

Cholesterol: oxygen oxidoreductase

含 量

≥50 units/mg protein, recombinant, expressed in E. coli

CAS  NO.

9028-76-6

MDL  number

MFCD00130783

适用范围

生物试剂

l  理化信息

外 观

淡黄色至深黄色冻干粉末

物理形态

Supplied as a lyophilzed powder containing sucrose

l  生物学信息

生物活性/药理作用

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H²O².

应 用

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.Cholesterol oxidase is used to determine serum cholesterol.The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

样品准备说明

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.5. Prepare solutions immediately before use.

有效单位定义

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov