产品名称

D-Fructose Dehydrogenase from Gluconobacter industrius

别 称

D-Fructose:(acceptor) 5-oxidoreductase

含 量

400-1,200 units/mg protein

CAS  NO.

37250-85-4

MDL  number

MFCD00130905

适用范围

生物试剂

l  理化信息

外 观

粉橙色冻干粉

物理形态

Lyophilized powder containing citrate-phosphate buffer salts, TRITON® X-100, and stabilizer

l  生物学信息

生物活性/药理作用

D-fructose dehydrogenase catalyzes the oxidation of D-fructose to 5-keto-D-fructose.Fructose dehydrogenase (FDH) is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca.140 kDa, consisting of subunits I (67kDa), II (51kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

应 用

D-fructose dehydrogenase is used as a biosensor to detect the presence of D-fructose.Fructose dehydrogenase (FDH)  is used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis.D-fructose dehydrogenase has been used in a study that contributed towards a convenient method for measuring rare sugars, monosaccharides, for applications in the bio-industry. A direct electron transfer reaction of d-fructose dehydrogenase adsorbed on a porous carbon electrode surface has been used to describe a batch-type coulometric d-fructose biosensor.

有效单位定义

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov