产品名称

Catalase from Aspergillus niger

别 称

H²O²:H²O² oxidoreductase

含 量

≥4,000 units/mg protein

CAS  NO.

9001-05-2

分子量

tetramer mol wt ~250 kDa

MDL  number

MFCD00081483

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 6.0

l  生物学信息

生物活性/药理作用

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds  NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme  activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

应 用

Catalase from Sigma has been used as a positive control during the functional characterization of Clostridium difficile spore coat proteins.

注 意

Freezing of solutions is not recommended.

有效单位定义

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of  A²⁴⁰.

l  包装与存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov