产品名称

Catalase from bovine liver

一般描述

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.

别 称

H²O²:H²O² oxidoreductase

含 量

2,000-5,000 units/mg protein; suitable for cell culture

CAS  NO.

9001-05-2

分子量

tetramer mol wt ~250 kDa

MDL  number

MFCD00081483

适用范围

生物试剂

l  理化信息

外 观

深绿色至非常深绿色，深米色至深褐色至深棕色粉末

l  生物学信息

生物活性/药理作用

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H²O² molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

应 用

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids,  and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver is used for the following applications:

• Gel filtration

• Analytical filtration on Ultrogel and glycerol gradient centrifugation

• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor

• Enzyme assays

• Alcohol oxidase activity

样品准备说明

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM  potassium phosphate buffer at 1mg/mL and pH 7.0.     

注 意

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

有效单位定义

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A²⁴⁰.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov