产品名称

Catalase from bovine liver

别 称

H²O²:H²O² oxidoreductase

含 量

≥10,000 units/mg protein

CAS  NO.

9001-05-2

分子量

tetramer mol wt ~250 kDa

MDL  number

MFCD00081483 

适用范围

生物试剂

l  理化信息

外 观

冻干粉

溶解性(25°C)

50 mM potassium phosphate buffer, pH 7.0: soluble 2.0 mg/mL

l  生物学信息

生物活性/药理作用

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H²O² molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

Catalase activity is constant over the pH range of 4.0-8.5. The enzyme activity is inhibited by 3-amino-1-H-1,2,4  triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

应 用

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene and apoptosis.  It has also been used to protect against oxidative damage to proteins,  lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

注 意

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

有效单位定义

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of  A²⁴⁰.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov