产品名称

Catalase from human erythrocytes

别 称

H²O²:H²O² oxidoreductase

含 量

≥90% (SDS-PAGE);  ≥30,000 units/mg protein

CAS  NO.

9001-05-2

分子量

tetramer mol wt ~250 kDa

MDL  number

MFCD00081483

适用范围

生物试剂

l  理化信息

外 观

缓冲水溶液

物理形态

Solution in 50 mM Tris, pH 8.0

l  生物学信息

生物活性/药理作用

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It  functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids.Catalase has also been used to study the role reactive oxygen species play in gene and apoptosis.

Each subunit of the tetramer contains iron  bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4  triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

有效单位定义

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A²⁴⁰.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov