产品名称

Catalase−Agarose enzyme from bovine liver

别 称

H²O²:H²O² Oxidoreductase

含 量

1900-3800 units per ml of agarose

分子量

tetramer mol wt ~250 kDa

MDL  number

MFCD00081483

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 3.2 M (NH⁴)²SO⁴, pH 7, containing 0.02% thimerosal

l  生物学信息

生物活性/药理作用

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close  proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu₂₊ results in degradation of the catalase molecule.

应 用

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H²O²) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing.Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H²O²) that can degrade the target product.

注 意

Freezing of solutions is not recommended.

有效单位定义

One unit will decompose 1.0 μmole of H²O² per min at pH 7.0 at 25 °C, while the H²O² concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of  A²⁴⁰.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov