产品名称

Chloroperoxidase from Caldariomyces fumago

别 称

Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase

含 量

≥3,000 units/mL

CAS  NO.

9055-20-3

MDL  number

MFCD00130774

适用范围

生物试剂

l  理化信息

外 观

缓冲水悬浮液

物理形态

Crude suspension in 0.1 M sodium phosphate,  pH ~4.5

l  生物学信息

生物活性/药理作用

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H²O² decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including  polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. It is similar magnetic and spectroscopic properties of cyctochrome P-450. Chloroperoxidase can catalyze the halogenations of organic substrates.

应 用

A useful alternative to lactoperoxidase for₁₃₁I ion labeling studies, for bromination of proteins, and for ₃₆Cl  labeling of macromolecules in long-term isolation procedures.

Chloroperoxidase is a useful alternative to lactoperoxidase for ₁₃₁I ion labeling studies, for bromination of proteins, and for ₃₆Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors.It has been used to study biocatalytic oxidation in polymersome nanoreactors.

有效单位定义

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H²O².

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov