产品名称

Peroxidase from horseradish

一般描述

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose,  mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca₂₊ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

别 称

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

含 量

200-300 units/mg solid (using pyrogallol) Highly stabilized, essentially salt-free

CAS  NO.

9003-99-0

MDL  number

MFCD00071339

适用范围

生物试剂

l  理化信息

外 观

棕色到非常深棕色，红色到非常深红色和红棕色冻干粉末

l  生物学信息

生物活性/药理作用

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol.

应 用

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism.It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme.

样品准备说明

Stabilized by chemical protection of the primary amines.

注 意

Maintains activity at low pH and higher temperature.

有效单位定义

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov