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Peroxidase from horseradish

商品编号: S0098
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  • S0098-5KU

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    553

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    S0098-25KU

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    1404

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    S0098-50KU

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    2561

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    S0098-100KU

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    4420

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    S0098-200KU

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    7852

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国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

Peroxidase from horseradish

一般描述

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose,  mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400  Daltons). At least seven isozymes of HRP exist. The isoelectric point for  horseradish peroxidase isozymes ranges from 3.0 - 9.0.

别 称

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

含 量

Type I, 50-150 units/mg solid (using  pyrogallol);essentially salt-free

CAS  NO.

9003-99-0

MDL  number

MFCD00071339

适用范围

生物试剂

l  理化信息

外 观

棕色和棕红色和红棕色至非常深红棕色基本上不含盐冻干粉末

溶解性(25°C)

0.1 M phosphate buffer: soluble (pH 6.0) H2O: soluble

l  生物学信息

生物活性/药理作用

HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2]  complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including  glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-ers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase.Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+,  Co2+, Cu2+, Fe3+, Mn2+, Ni2+,  Pb2+ ions are known to inhibit the enzyme activity.

When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification.

有效单位定义

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov