Phone:020-31155029| Tech Support:tech@tomums.cn| E-mail:sales@tomums.cn
首页 > 产品 > 生命科学 > 酶和蛋白 > Peroxidase from horseradish

Peroxidase from horseradish

商品编号: S0099
图示 货号 库存 价格(¥) 数量
  • S0099-5KU

    有货

    776

    - +

    S0099-25KU

    有货

    2548

    - +

    S0099-50KU

    有货

    3575

    - +

    S0099-100KU

    有货

    6240

    - +

    S0099-200KU

    有货

    10738

    - +

备注:

国外库存,货期2-4周,具体详情请联系销售人员。

质量文档

详细介绍

l  基本信息

产品名称

Peroxidase from horseradish

一般描述

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

别 称

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

含 量

Type II, 150-250 units/mg solid (using pyrogallol) ;essentially salt-free

CAS  NO.

9003-99-0

MDL  number

MFCD00071339

适用范围

生物试剂

l  理化信息

外 观

棕色到非常深棕色和红色到非常深红色基本上无盐冻干粉末

溶解性(25°C)

0.1 M phosphate buffer: soluble (pH 6.0) H2O:  soluble

l  生物学信息

生物活性/药理作用

HRP readily combines with hydrogen peroxide  (H2O2) and the resultant [HRP-H2O2]  complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including  glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-ers. It is smaller and more stable than  the enzyme labels β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+,  Co2+, Cu2+, Fe3+, Mn2+, Ni2+,  Pb2+ ions are found to inhibit the enzyme activity. When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant.

应 用

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Horseradish peroxidase, product P8250, has been used to study nonoral antigens in inflamed gingiva and Ebola virus glycoprotein toxicity. The enzyme has been used as a comparison during the peroxidase assay of extract from mature tall  fescue leaf blades. It has also been used to measure H2O2production.

有效单位定义

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov