产品名称

Lipoxidase from Glycine max (soybean)

别 称

Linoleate:oxygen oxidoreductase, Lipoxygenase

含 量

500,000-1,000,000 units/mg protein

CAS  NO.

9029-60-1

分子量

mol wt 108 kDa (two 54 kDa subunits)

MDL  number

MFCD00131527

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 2.3 M (NH⁴)²SO⁴ solution, pH approx. 6.0

l  生物学信息

生物活性/药理作用

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

应 用

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE). Lipoxidase from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

样品准备说明

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

有效单位定义

One unit will cause an increase in A234 of  0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1cm light path). One A234 unit is equivalent to the oxidation of  0.12 μmole of linoleic acid.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov