产品名称

Protocatechuate 3,4-Dioxygenase from Pseudomonas sp.

含 量

≥3 units/mg solid

CAS  NO.

9029-47-4

分子量

mol wt ~700 kDa

MDL  number

MFCD00132132

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Supplied as lyophilized powder containing approx. 40% stabilizer.

l  生物学信息

应 用

Protocatechuate 3,4-Dioxygenase(PCD), from  Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the bolism of protocatechuate in Rhizobiaceae. The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow ing and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

有效单位定义

One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov