l 基本信息 |
产品名称 | Nitric Oxide Synthase, Inducible from mouse |
一般描述 | Inducible nitric oxide synthase (iNOS), also known as inflammatory nitric oxide synthase, is a calcium independent isoenzyme, involved in synthesis of nitric oxide (NO). It is a soluble enzyme encoded by the gene mapped to mouse chromosome 11. iNOS is active in dimeric form and its activity is induced by cytokines and various other stimuli. iNOS is expressed in various inflammatory conditions. |
别 称 | Inducible Nitric Oxide Synthase, NOS II, iNOS, macNOS |
含 量 | Protein by Bradford: ≥1mg/mL; Enzymatic Activity: ≥4.0 UN/mg; recombinant, expressed in E. coli |
分子量 | mol wt 130 kDa (homodimer) | MDL
number | MFCD00803363 |
适用范围 | 生物试剂 |
l 理化信息 |
外 观 | 缓冲水溶液 |
物理形态 | Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 8 μM tetrahydrobiopterin |
l 生物学信息 |
生物活性/药理作用 | NOS is responsible for the biosynthesis of
nitric oxide from L-arginine. iNOS is not calcium/calmodulin dependent and has a Km = 16 μM for L-arginine. Tumor-derived inducible nitric oxide synthase (iNOS) plays a vital role in stimulating tumor growth and vessel maturation. Therefore, it is considered to be a potential therapeutic target for anti-vascular cancer therapies. Unchecked activity of iNOS leads to overproduction of nitric oxide (NO), which is toxic for living cells. iNOS activity can be controlled at both tranion and translational level by regulating protein stability, dimerization, phosphorylation, cofactor binding and availability of oxygen and L-arginine as substrates. iNOS plays a vital role in excisional wound repair and exhibits gene therapy strategy to advance wound healing process in iNOS-deficient conditions such as diabetes and
steroid treatment. |
应 用 | Nitric Oxide Synthase, Inducible from mouse has been used in immunohistochemical studies. It is also used to evaluate the therapeutic efficacy of inducible nitric oxide synthase (NOS) on reperfusion-induced microcirculatory alterations and hemodynamic adverse effects in the microvasculature of skeletal muscle. |
有效单位定义 | One unit will produce 1.0 μmol of nitric
oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 0.15 mM NADPH, 4.5 μM oxyhemoglobin, 18 μM
tetrahydrobiopterin and 180 μM DTT. |
l 存储 |
存储温度 | The product should be stored at –70 °C. It
will remain active for at least 6 months at that temperature. The enzyme
loses ~10% of its activity after a single freeze-thaw cycle. Therefore, after
the initial defrost, it is recommended the product be stored in single-use
aliquots at –70 °C. During use, keep the solution on ice at all times since
the enzyme loses activity at higher temperatures. |
l 注意事项及免责声明 |
本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。 |
l 参考文献 |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
