产品名称

Cyclooxygenase 1 from sheep

别 称

COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase

含 量

≥1500 units/mg protein

分子量

dimer subunit mol wt 70 kDa

MDL  number

MFCD02098124

适用范围

生物试剂

l  理化信息

外 观

甘油溶液

物理形态

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN_® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

l  生物学信息

生物活性/药理作用

COX-1 catalyzes the conversion of  arachidonic acid to prostaglandin H² (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K^M of 8.3 μM for arachidonic acid.

有效单位定义

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

l  包装与存储

存储温度

The enzyme should be stored at –70 °C. After initial defrost, it is recommended the product be aliquoted into tubes and re-frozen at –70 °C to avoid repeated freeze-thaw cycles. Do not store in a frost-free freezer.

COX-1 is relatively unstable at room temperature; to prevent loss of activity during experiments, keep the enzyme on ice (2–8 °C) at all times.

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov