产品名称

Superoxide Dismutase from bovine liver

一般描述

Superoxide dismutases are a group of low molecular weight lloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O²-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

别 称

SOD, Superoxide: superoxide oxidoreductase

含 量

2,000-6,000 units/mg protein (biuret)

CAS  NO.

9054-89-1

MDL  number

MFCD00132404

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 3.8 M (NH⁴)²SO⁴, pH 7.0

l  生物学信息

生物活性/药理作用

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell  lines, and transgenic mice.

应 用

Superoxide dismutase from bovine liver has been used in a study to assess the inactivation of endothelial derived relaxing factor by oxidized lipoproteins. Superoxide dismutase from bovine liver has also been used in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

样品准备说明

SOD is soluble in water (20 mg/ml) yielding a colorless to blue-green solution. SOD is also soluble in aqueous buffers such as 0.1 M potassium phosphate, pH 7.5.

有效单位定义

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA⁵⁵⁰ of 0.025 ± 0.005 per min.

l  包装与存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov