产品名称

Superoxide Dismutase bovine

一般描述

SOD from bovine erythrocytes was the first SOD to be found in mammalian tissues. Before its enzymatic activity was discovered the protein was known as haemocuprein or erythrocuprein. SOD from bovine erythrocytes is a homodimeric non-covalently bound protein with two 16.3 kDa subunits of 151 amino acids. Each monomer has one intrachain disulfide and one free sulfhydryl, one Cu₊₂ atoms and one Zn⁺² atoms. There are three forms of SOD differentiated by the l ions in the active site. These are Cu₊₂/Zn₊₂, Mn₊₂, and  Fe₊₂ SOD. In vertebrate organisms Cu/Zn-SOD is found in the cytoplasm and the mitochondrial intermembrane space, while Mn-SOD is found in the mitochondrial matrix space. Fe-SOD is found in prokaryotes and some  higher plants.

别 称

CU/ZN-SOD, SOD1, SOD, Superoxide Dismutase 1 bovine, Superoxide: superoxide oxidoreductase

含 量

≥2500 units/mg protein, ≥90% (SDS-PAGE);  recombinant, expressed in E. coli

CAS  NO.

9054-89-1

MDL  number

MFCD00132404

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.

应 用

Superoxide dismutase has been used in a study to investigate where lipoproteins may affect the L-arginine-nitric oxide pathway. Superoxide dismutase has also been used in a study to investigate the mass spectral evidence for carbonate-anion-radical-induced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase.The product has been used to develop an SOD assay. This assay used dismutase-mediated inhibition of NADH-dependent nitroblue tetrazolium reduction.

样品准备说明

Produced using animal component-free materials.

有效单位定义

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0  ml reaction volume. Xanthine oxidase concentration should produce an initial  ΔA⁵⁵⁰ of 0.025 ± 0.005 per min.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov