产品名称

Aldehyde Dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae)

别 称

Aldehyde:NAD[P]₊ oxidoreductase

含 量

≥2.0 units/mg protein

CAS  NO.

9028-88-0

MDL  number

MFCD00081306

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Contains lactose, potassium phosphate and citrate buffer salts, and mercaptosuccinic acid.

l  生物学信息

生物活性/药理作用

Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme’s activity. Rubidium or NH4₊ can be substituted for K₊, and glutathione for cysteine. Lithium, Na₊, and Cs₊ inhibit the reaction.  Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.

应 用

Aldehyde dehydrogenase from baker’s yeast catalyzes the reduction of pyridine nucleotides by several aldehydes. It catalyzes the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. The enzyme has been used to study the production of ethanol and isobutanol. Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of NAD using alcohol dehydrogenase after preremoval of aldehyde by aldehyde dehydrogenase. Aldehyde dehydrogenase (ALDH) from Sigma has been used to evaluate the effects of pear extracts on ALDH activity during the study of the effects and action mechanisms of korean pear on alcohol detoxification.

样品准备说明

This enzyme is soluble in 100 mM Tris HCl buffer,pH 8.0, containing 0.02% BSA (0.3 mg/ml), yielding a clear, colorless solution.

有效单位定义

One unit will oxidize 1.0 μmole of acetaldehyde to acetic acid per min at 25 °C at pH 8.0 in the presence of β-NAD₊, potassium and thiols.

l  包装与存储

存储温度

-20 °C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov