产品名称

Pyruvate Oxidase from microorganisms

别 称

Pyruvate:oxygen oxidoreductase (phosphorylating)

含 量

≥1.5 U/mg

CAS  NO.

9001-96-1

分子量

mol wt ~260 kD

MDL  number

MFCD00132148

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing FAD and sugar as stabilizer

l  生物学信息

生物活性/药理作用

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

应 用

Pyruvate Oxidase (PoxB) converts pyruvate  directly to acetate and CO². It is used to study pyruvate bolism. It is used to study aerobic bolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae. Pyruvate Oxidase is used  for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.

有效单位定义

One unit will produce 1.0 μmole of H²O² per min during the conversion of pyruvate and phosphate to acetylphosphate and CO² at pH 5.7 at 37 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov