产品名称

L-Alanine Dehydrogenase from Bacillus subtilis

别 称

L-Alanine: NAD₊ oxidoreductase (deaminating)

含 量

~30 units/mg protein (Lowry)

CAS  NO.

9029-06-5

MDL  number

MFCD00131441

适用范围

生物试剂

l  理化信息

外 观

澄清至略微浑浊无色至黄色缓冲的甘油水溶液

物理形态

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

l  生物学信息

生物活性/药理作用

L-Alanine dehydrogenase is an  A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent l ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

应 用

L-Alanine dehydrogenase converts L-alanine  to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may  be used to study enzyme inactivation and protection.

有效单位定义

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH³ per min at pH 10.0 at 25 °C.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov