产品名称

L-Glutamic Dehydrogenase from bovine liver

别 称

L-GLDH, L-Glutamate:NAD[P]₊ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver

含 量

≥35 units/mg protein, Type II

CAS  NO.

9029-12-3

MDL  number

MFCD00131461

适用范围

生物试剂

l  理化信息

外 观

甘油水溶液

物理形态

Solution in 50% glycerol, pH 7.3

l  生物学信息

生物活性/药理作用

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian bolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:

•  It has a reversible concentration-dependent association, producing higher molecular weight forms.

•  Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.

•  Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

应 用

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.

样品准备说明

This product is supplied as a solution in 10 mM phosphate buffer, pH 7.0, containing 50% glycerol. This solution is  then diluted to 0.3 - 0.6 units/ml using triethanolamine buffer, pH 7.3, for  use in enzymatic assays.

有效单位定义

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of  ammonium ions.

l  包装与存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov