产品名称

L-Amino Acid Oxidase from Crotalus adamanteus

别 称

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

含 量

≥0.3 unit/mg solid, Type I

CAS  NO.

9000-89-9

MDL  number

MFCD00131454

适用范围

生物试剂

l  理化信息

外 观

固体

溶解性(25℃)

H₂O: soluble 1.0 mg/mL, clear(lit.)

l  生物学信息

生物活性/药理作用

L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver. The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various bolic pathways such as alanine and aspartate bolism, methionine bolism, valine, leucine and isoleucine degradation, tyrosine bolism, phenylalanine bolism, tryptophan bolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis.

应 用

L-amino acid oxidase (LAAO) is used to  convert L-amino acids to their corresponding α-keto acids. One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37°C. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

样品准备说明

Dissolves in water at 1 mg/mL concentration to form a clear solution.

有效单位定义

One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37°C. (L-Leucine is deaminated at the same rate at pH 7.8 at 37 °C.)

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov