产品名称

Diamine Oxidase from porcine kidney

别 称

Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)

含 量

≥0.05 unit/mg solid

CAS  NO.

9001-53-0

MDL  number

MFCD00130946

适用范围

生物试剂

l  理化信息

外 观

固体

溶解性(25℃)

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

l  生物学信息

生物活性/药理作用

Diamine oxidase from porcine kidney is a homodimer consisting of 2 equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.The molecular mass of the enzyme is found to be 170 kDa.The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. It catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen bolism. It is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.

应 用

Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-d test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-ed oligosaccharide structures in diamine oxidase.

样品准备说明

This enzyme is soluble in 100 mM sodium phosphate buffer, pH 7.2 (10 mg/ml).     

有效单位定义

One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov