产品名称

Cytochrome P450 Reductase human

一般描述

This human, recombinant protein is isolated from insect cells infected with a baculovirus containing the cDNA for human  cytochrome P450 reductase. It is purified by affinity chromatography.

别 称

NADPH: Ferrihemoprotein oxidoreductase

含 量

Purity by SDS Electrophoresis:≥90%; ≥1.0mg protein/ml (Bradford); Enzymatic Activity: ≥30units/mg; recombinant, expressed in baculovirus infected insect cells

分子量

calculated mol wt 76.5 kDa

MDL  number

MFCD01093329

适用范围

生物试剂

l  理化信息

外 观

甘油溶液

物理形态

Supplied in a solution containing 10 mM potassium phosphate, pH 7.4, 0.1 mM EDTA, 0.5 mM DTT, 20% (v/v) glycerol

l  生物学信息

生物活性/药理作用

Cytochrome P450 reductase is a membrane bound enzyme required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. The cytochrome P450 enzyme system is mainly involved in the detoxification of xenobiotics in the liver.It also participates in the activation of procarcinogens and the bolism of endogeneous substrates such as steroids.

应 用

NADPH-P450 reductase is a membrane-bound  flavoprotein that transfers electrons from NADPH to the various isoforms of cytochrome P450. The ratio of reductase:P450 that is typically used ranges from 0.5-5:1. The enzyme contains one mole each of FAD and FMN per mole of protein.Human cytochrome P450 reductase has been used in a study to assess the biocatalytic synthesis and structure elucidation of cyclized bolites of the deacetylase inhibitor panobinostat (LBH589). Human cytochrome P450 reductase has also been used in a study to investigate the effects of coupled motions on electrons along the human microsomal P450 chains.

The enzyme from sigma has been used in the hypoxic and oxic reductions of the anticancer prodrug, 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)-2-[[1-(4-nitrophenyl)ethoxy]carbonyl]hydrazine  (KS119).

有效单位定义

One unit will cause the reduction of 1.0 μmole of cytochrome c by NADPH per minute at pH 7.4 at 37 °C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov