产品名称

Glutathione Reductase from baker's yeast (S. cerevisiae)

一般描述

Glutathione reductase (GLR1) exists in mitochondrial and cytoplasmic isoforms. It shares sequence and structural homology to thioredoxin reductase, and is a flavin-containing oxidoreductase. Its  active site is composed of a redox-active disulphide, and it requires NADPH  for its catalytic activity. It is a widely present enzyme and is found in plants,bacteria, yeast, mice and humans.

别 称

GR, NAD(P)H:oxidized-glutathione  oxidoreductase

含 量

100-300 units/mg protein (biuret)

CAS  NO.

9001-48-3

分子量

mol wt 118 kDa

MDL  number

MFCD00131196

适用范围

生物试剂

l  理化信息

外 观

淡黄色至深黄色硫酸铵悬浮液

物理形态

Suspension in 3.6 M (NH4)2SO4, pH 7.0, containing 0.1 mM dithiothreitol

l  生物学信息

生物活性/药理作用

Glutathione (γ-glutamylcysteinylglycine) is a ubiquitous tripeptide thiol which plays a crucial role in oxidative stress defence mechanism of the cell. Glutathione reductase (GLR1) is responsible for the reduction of the glutathione disulfide (GSSG) to reduced glutathione  (GSH). Glutathione reductase IGR) is a crucial flavoenzyme in the antioxidant defense system. Reduced glutathione (GSH) is used by glutathione peroxidase  to detoxify hydrogen peroxide and in the process is converted to oxidized glutathione (GSSG). The GSSG is then recycled back to GSH by glutathione reductase (GR) using NADPH that is then converted to NADP⁺. The regenerated GSH is then available to detoxify more hydrogen peroxide. The enzyme uses FAD as a cofactor. GR and glutathione peroxidase may inhibit lipid peroxidation by functioning as antioxidant enzymes in sperm. Glutathione reductase shares a structural motif with a number of other proteins including aspartyl proteases, citrate synthase, EF hands, hemoglobins, lipocalins, and α/β hydrolases. GR is stimulated by melatonin and is reportedly irreversibly inhibited by a number of oxygen radical generating systems.

应 用

Glutathione Reductase (GR) from baker′s yeast has been used:

• in the glutathione assay to determine glutathione concentration.

• as a standard in the generation of calibration curve.

• as an antigen to measure plasma activity of GR.

Glutathione reductase (GR) from baker′s yeast (Saccharomyces cerevisiae) has been used-:• for quantifying the myocardial tissue glutathione content using a glutathione  reductase-5,5′-dithiobis (2-nitrobenzoic acid)-d enzymatic recycling assay

• for the quantification of reduced glutathione (GSH) in the oocytes, using a slightly modified microglutathione assay, obtained from prepubertal gilts

• for the preparation of total GSSG (glutathione disulphide) + GSH measurement, where all available GSSG was reduced to GSH, in rat lens

• for the quantification of intracellular reduced glutathione (GSH) in the oocytes obtained from rats

样品准备说明

Purified by affinity chromatography

有效单位定义

One unit will reduce 1.0 μmole of oxidized  glutathione per min at pH 7.6 at 25 °C.

l  包装与存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov