产品名称

Thioredoxin Reductase from Escherichia coli

一般描述

NADPH:oxidized thioredoxin oxidoreductase

别 称

NADPH:oxidized thioredoxin oxidoreductase

含 量

>25 units/mg protein (Bradford)

MDL  number

MFCD00678149

适用范围

生物试剂

l  理化信息

外 观

灰白色至黄色硫酸铵悬浮液

物理形态

Suspension in 3.6 M (NH4)2SO4 containing 30mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.

l  生物学信息

生物活性/药理作用

Thioredoxin reductase (TrxR) is an  NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide. An FAD-containing enzyme involved in the transfer of hydrogen from E. coli thioredoxin to other proteins thus providing a powerful disulfide reductase system.Thioredoxin reductase is a FAD containing enzyme, which transfers the reducing equivalent from NADPH to the disulphide bond of the enzyme by using FAD moiety within the Cys-Ala-Thr-Cys sequence. It can also reduce Trx-S2 to Trx-(SH)2 by using NADPH.

应 用

Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the  peroxidase activitiy of Cys-d thiol peroxidases. The product was used for determining the enzymatic activity of His6-Ahp1p.

有效单位定义

One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E.coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov