产品名称

Thioredoxin Reductase from rat liver

别 称

NADPH:Oxidised Thioredoxin Oxidoreductase, Thioredoxin: NADP₊ Oxidoreductase

含 量

≥100 units/mg protein (Bradford)

CAS  NO.

9074-14-0

MDL  number

MFCD00678149

适用范围

生物试剂

l  理化信息

外 观

无色至淡黄色缓冲的甘油水溶液

物理形态

Solution in 50 mM Tris-HCl, pH 7.5, 300 mM  NaCl, 1 mM EDTA, and 10% glycerol.

l  生物学信息

生物活性/药理作用

Thioredoxin reductase (TrxR) is a NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidized thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as non-disulfide substrates such  selenite, lipoic acids, lipid hydroperoxides,and hydrogen peroxide. Thioredoxin  reductase from mammalian sources contains a selenocysteine residue that is essential for the activity of the enzyme. It is one of the antioxidant enzymes present in the mammalian cell together with catalase, glutathione peroxidase and superoxide dismutase, and helps in removal of reactive oxygen species (ROS) from the cell. An example is the removal of excess nitric oxide (NO) by the formation of a complex with glutathione forming the S-nitroso-glutathione adduct (GS-NO). This can be cleaved directly by thioredoxin reductase. Hydrogen peroxide, another deleterious oxidant in the cell, is also reduced directly by mammalian TrxR.

应 用

Thioredoxin Reductase from rat liver can be used for studying the uptake and reduction of a-lipoic acid by utilizing reducing capacity of human erythrocytes. The product can also be used for studying the activation mechanism of transglutaminase 2 (TG2) in the extracellular matrix by using Thioredoxin.

有效单位定义

One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a non-coupled assay containing DTNB alone as substrate) per minute at pH 7.0 at 25 °C.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov