产品名称

Transglutaminase from guinea pig liver

别 称

Protein-glutamine:amine γ-glutamyltransferase, Protein-Glutamine-γ-Glutamyltransferase

含 量

≥1.5 units/mg protein

CAS  NO.

80146-85-6

MDL  number

MFCD00132446

适用范围

生物试剂

l  理化信息

外 观

白色至灰白色冻干粉末

物理形态

Lyophilized powder containing Tris and dithioerythritol

溶解性(25℃)

H²O: soluble 1.0 mg/mL, clear

l  生物学信息

生物活性/药理作用

Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross ing of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

应 用

This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.

样品准备说明

This enzyme is soluble in water (1 mg/ml), yielding a clear solution.

有效单位定义

One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at  37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

l  包装与存储

存储温度

-20 °C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov