产品名称

Dextran Sucrase from Leuconostoc mesenteroides

含 量

≥100 units/mg protein

CAS  NO.

9032-14-8

MDL  number

MFCD00130936

适用范围

生物试剂

l  理化信息

外 观

白色至淡黄色冻干粉末

物理形态

Lyophilized powder containing dextran, MES buffer salts and CaCl²

溶解性(25°C)

H²O: soluble 0.9-1.1 mg/mL

l  生物学信息

生物活性/药理作用

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer ed mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching ages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.

应 用

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been  used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.

有效单位定义

One unit will liberate 1.0 μmole of  fructose per min at 37 °C, pH 5.4.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov