产品名称

Nucleoside Phosphorylase from microorganisms

一般描述

Nucleoside phosphorylase is an enzyme important for the biosynthesis of nucleosides.

别 称

Nucleoside Phosphorylase bacterial, PNP,  Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

含 量

≥10 units/mg protein

CAS  NO.

9030-21-1

分子量

mol wt ~120 kDa

MDL  number

MFCD00131739

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing potassium gluconate, mannitol and EDTA

l  生物学信息

生物活性/药理作用

Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

应 用

Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation. This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase (XTO-212) and uricase (UAO-201, UAO-211). Purine nucleoside phosophorylase has shown the ability to perform both phosphorylosis and synthesis of purine deoxy- and  ribonucleosides.It has also been found that membrane-associated nucleoside phosphorylases may have a transmembranal orientation with their and ribose-1-P binding sites on opposite sides of the membrane.

有效单位定义

One unit will cause the phosphorolysis of 1.0 μmole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.4  at 25 °C.

l  包装与存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov