产品名称

PARP-1 human (recombinant, expressed in E. coli)

一般描述

Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis.

别 称

NAD₊ ADP-ribosyltransferase, Poly(ADP-ribose) Polymerase

MDL  number

MFCD01324157

适用范围

生物试剂

l  理化信息

物理形态

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1% Triton™-X 100, 50 % glycerol, and 0.1 mg/ml BSA.

l  生物学信息

生物活性/药理作用

PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis.PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn₂₊ and DNA, and recognizes single-strand  breaks in DNA. It is involved in excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive  HIV-1 infection.

应 用

PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD₊, specifically binds Zn₂₊ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments.

有效单位定义

One unit will incorporate 10 femptomole of poly(ADP-ribose) from NAD into 5 μg of immobilized histone proteins in 30 minutes at 22 °C in a 96 well plate.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov