产品名称

Thymidine Phosphorylase, recombinant from Escherichia coli

一般描述

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

别 称

Thymidine:orthophosphate deoxy-D-ribosyltransferase

含 量

recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

CAS  NO.

9030-23-3

MDL  number

MFCD00132426

适用范围

生物试剂

l  理化信息

外 观

无色至黄色液体

物理形态

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

l  生物学信息

生物活性/药理作用

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine s to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

应 用

Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma.

样品准备说明

Cloned from E. coli and produced in overexpressing E. coli

有效单位定义

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov