产品名称

Nucleoside Deoxyribosyltransferase II from Lactobacillus leichmanii

别 称

DRTase, Deoxyribose transferase, NDT,  Nucleoside Deoxyribosyltransferase II from Lactobacillus leichmanii, nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase

含 量

recombinant, expressed in E. coli; ≥80% protein basis (biuret); ≥1.0 units/mg protein

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Enzymatic nucleoside synthesis is an attractive alternative to traditional chemical methods which require multiple chemical reactions and the use of chemicals that are both expensive and environmentally harmful. Class II Nucleoside Deoxyribosyltransferases exhibit multiple characteristics that make them suitable as biocatalysts for the synthesis of natural and nonnatural nucleosides. They are specific for 2′-deoxyribonucleosides, regioselective (N-1 glycosylation in pyrimidine and N-9 in purine), and stereoselective (forming only β-anomers). Class II N-Deoxyribosyltranferases, DRTases, catalyze the transfer of a 2′-deoxyribosyl group between purines or pyrimidines. In the absence of an acceptor nucleo, these enzymes display hydrolase activity, converting the nucleoside to its and a deoxyribose. In lactobacilli species, Nucleoside Deoxyribosyltransferase enzymes are part of the nucleoside salvage pathway for DNA synthesis.

应 用

Nucleoside deoxyribosyltransferase II has been used in a study that assessed its enzymatic synthesis with 2′-deoxyguanosine. Nucleoside deoxyribosyltransferase II has also been used in studies to investigate its molecular cloning, and specificity.

样品准备说明

Produced using animal component-free materials.

有效单位定义

One unit of enzyme produces 1 μM of hypoxanthine in 1 minute at 40°C, pH 6.0.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov