产品名称

α-2,3-Sialyltransferase from Pasteurella multocida

一般描述

Sialyltransferases belongs to the glycosyltransferases family with the capability of catalyzing the transfer of N-acetylneuraminic acid residues. It is a multifunctional enzyme with α-2,3-sialyltransferase activity, α-2,6-sialyltransferase activity, sialidase activity, and trans-sialidase activity. It has a molar mass of 46.4 kDa, with pI, pH being 5.94 and 7.5-8.5 respectively.

别 称

CMP-N-acetylneuraminate:β-D-galactoside α-(2,3)-N-acetylneuraminyltransferase

含 量

≥2 units/mg protein; recombinant, expressed in E. coli BL21

MDL  number

MFCD00130470

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing Tris-HCl and NaCl

l  生物学信息

生物活性/药理作用

Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides. Sialyltransferase catalyzes the transfer of Neu5Ac from CMP-Neu5Ac as a donor substrate to the β-D-galactosyl-1,4-N-acetyl-D-glucosaminyl termini of acceptor molecules including glycoproteins, glycolipids,and oligosaccharides.

样品准备说明

Reconstitute the lyophilized powder with water to ~5 mg/mL. Solutions can be stored at 2-8°C for 1-2 months after reconstitution. They can also be aliquoted and frozen at -70°C or -20°C for 1year. Multiple freeze-thaw cycles should be avoided.

有效单位定义

One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C  at pH 8.0.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov