产品名称

Glutathione S-Transferase from human placenta

一般描述

Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes.Cytoplasmic isoforms of GST are encoded by five genes, namely α, γ, μ, σ and  π. α, μ and π are the most abundant forms in mammals. GSTπ is the isoform of this enzyme found in placenta and is composed of 209 amino acids.

别 称

GST, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione  S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase

含 量

25-125 units/mg protein

CAS  NO.

50812-37-8

MDL  number

MFCD00131198

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA.

l  生物学信息

生物活性/药理作用

Glutathione S-transferase (GST) catalyzes conjugation reactions during phase II bolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles. Glutathione S-transferases are a family of proteins that catalyze the conjugation of  reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.

应 用

Glutathione S-Transferase from human placenta has been used for the immunization of rabbits for the production of specific anti-π-GST antibodies, to determine whether total anti-π-GST antibodies are more specific and reactive as compared to anti-peptide antibodies.

样品准备说明

Immediately before use, prepare a solution  containing 0.075–0.15 unit/ml of Glutathione S-Transferase in 100 mM  potassium phosphate buffer, pH 6.5, with 1.0 mM ethylenediaminetetraacetic  acid (EDTA). Long term storage of this dilute enzyme solution is not  recommended.

有效单位定义

One unit will conjugate 1.0 μmole of  1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at  25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov