产品名称

Glycerokinase from Cellulomonas sp.

别 称

ATP:glycerol 3-phosphotransferase, Glycerol Kinase

含 量

25-75 units/mg protein

CAS  NO.

9030-66-4

分子量

mol wt ~128 kDa ((by gel filtration))

MDL  number

MFCD00131205

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing phosphate buffer salts and sodium gluconate

l  生物学信息

生物活性/药理作用

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

应 用

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis.

有效单位定义

will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov