产品名称

Protein Kinase A from bovine heart

别 称

Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart

含 量

≥0.4 units/μg protein

CAS  NO.

9026-43-1

MDL  number

MFCD00132121 

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

l  生物学信息

生物活性/药理作用

Many 3′ ,5′ -cyclic  AMP dependent protein kinases have been reported. Structural studies (Traugh,  J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are ed together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

应 用

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.

样品准备说明

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

有效单位定义

One unit will transfer 1.0 picomole phosphate from γ-₃₂P-ATP to hydrolyzed and partially dephosphorylated casein per min at pH 6.5 at 30°C in the presence of cyclic AMP.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov