产品名称

Pyruvate Kinase from rabbit muscle

一般描述

Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.

别 称

ATP:pyruvate 2-O-phosphotransferase, PK

含 量

350-600 units/mg protein; Type II

CAS  NO.

9001-59-6

MDL  number

MFCD00081946

适用范围

生物试剂

l  理化信息

外 观

白色硫酸铵悬浮液

物理形态

Suspension in 3.2 M (NH⁴)²SO⁴ solution, pH 6

l  生物学信息

生物活性/药理作用

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Optimal Temperature: 25°C

ΕA²⁸⁰ = 0.54 for 1 mg(p)/ml,1 cm path

Reported K^M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH  7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of  ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn₂₊).

应 用

Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.

有效单位定义

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov