产品名称

Pyruvate Kinase from rabbit muscle

别 称

ATP:pyruvate 2-O-phosphotransferase, PK

含 量

350-600 units/mg protein; Type VII

CAS  NO.

9001-59-6

MDL  number

MFCD00081946

适用范围

生物试剂

l  理化信息

外 观

缓冲的甘油水溶液

物理形态

Solution in 50% glycerol containing 0.01M phosphate, pH 7.0

l  生物学信息

生物活性/药理作用

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Optimal Temperature: 25°C

ΕA²⁸⁰ = 0.54 for 1mg(p)/ml, 1cm path

Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH  7.4 and 30°C. Pyruvate kinase is highly specific for phosphoenolpyruvate,  but can utilize other dinucleotide triphosphates as substrates in place of  ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP. Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

应 用

Pyruvate kinase from Sigma has been used in  the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein . The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase.Pyruvate kinase is an enzyme involved in glycolysis and is involved in gluconeogenesis. Product P7768 is from rabbit muscle and is provided in a buffered aqueous glycerol solution and has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency.

有效单位定义

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov