产品名称

Pyruvate Kinase from rabbit muscle

别 称

ATP:pyruvate 2-O-phosphotransferase, PK

含 量

350-600 units/mg protein; Type III

CAS  NO.

9001-59-6

MDL  number

MFCD00081946 

适用范围

生物试剂

l  理化信息

外 观

白色冻干粉

l  生物学信息

生物活性/药理作用

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.

Isoelectric Point: 7.6

Optimal pH: ∼7.5

Optimal Temperature: 25°C

ΕA²⁸⁰ = 0.54 for 1mg(p)/ml, 1cm path

Reported K^M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30°C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of  ATP including GTP, ITP, dATP, UTP, and CTP. Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This  transfer yields one molecule of pyruvate and one molecule of ATP. The molecular weight is 237 kDa and it exists as a tetramer of four equal subunits of molecular weight 57 kDa. The isoelectric point is 7.6 and optimal  pH is around 7.5.

应 用

Pyruvate kinase is an enzyme involved in glycolysis and is involved in gluconeogenesis. Product P9136 is from rabbit muscle and is provided as a lyophilized powder. It has been used in plant spectrophotometric assays to measure ATP hydrolysis. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency.

样品准备说明

This enzyme is soluble in water (1 mg/ml), yielding a clear and colorless solution.

有效单位定义

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov