产品名称

Acetate Kinase from Bacillus stearothermophilus

别 称

ATP:Acetate phosphotransferase, Acetate Kinase Bacillus stearothermophilus

含 量

400-1,200 units/mg solid

CAS  NO.

9027-42-3

MDL  number

MFCD00130330

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Contains Tris-HCl buffer.

l  生物学信息

生物活性/药理作用

Involved in the bolism of propanoate, pyruvate and taurine. Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the bolism of propanoate, pyruvate and taurine.Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 %  α-helix and 43 % unordered structure.

应 用

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [₃₂P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [₃₂P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.

有效单位定义

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov